Mobilization of Calcium from Intracellular Stores, Potentiation of Neurotransmitter-Induced Calcium Transients, and Capacitative Calcium Entry by 4-Aminopyridine
نویسندگان
چکیده
منابع مشابه
Mobilization of calcium from intracellular stores, potentiation of neurotransmitter-induced calcium transients, and capacitative calcium entry by 4-aminopyridine.
In this study we analyzed the effect of 4-aminopyridine (4-AP) on free cytosolic calcium concentration ([Ca(2+)](i)) in basal conditions, after stimulation with neurotransmitters, and during capacitative calcium entry. Using fura-2 ratiometric calcium imaging, we found that 4-AP increased [Ca(2+)](i) in type I astrocytes, neurons, and in skeletal muscle cells. The [Ca(2+)](i) elevation induced ...
متن کاملCapacitative calcium entry.
The original idea that calcium might enter cells through a capacitative mechanism was first introduced by Jim Putney [1]. The somewhat surprising hypothesis was that calcium entry was regulated by the state of filling of the calcium stores. By analogy with a capacitor in an electrical circuit, the calcium stores prevent entry when they are charged up but immediately begin to promote entry as so...
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Capacitative Ca(2+) entry is a recently discovered Ca(2+) entry pathway that is activated on depletion of intracellular Ca(2+) stores, providing an avenue for store refilling. Despite recent progress in elucidating the capacitative Ca(2+) entry pathway, the mysteries of its molecular identity, its biophysical properties, and the store depletion signal remain.
متن کاملCapacitative calcium entry
A long-standing mystery in the cell biology of calcium channel regulation is the nature of the signal linking intracellular calcium stores to plasma membrane capacitative calcium entry channels. An RNAi-based screen of selected Drosophila genes has revealed that a calcium-binding protein, stromal interaction molecule (STIM), plays an essential role in the activation of these channels and may be...
متن کامل4-aminopyridine-induced contracture in frog ventricle is due to calcium released from intracellular stores.
The aim of the study is to demonstrate the presence of intracellular calcium store in frog ventricle based on contractures induced by 4-aminopyridine in calcium-free media. Frog-ventricular strips were subjected to field stimulation at 0.2 Hz and the force of contraction was recorded after stabilization. The preparation was then kept quiescent for some time in solutions with different sodium co...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 2001
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.21-09-03135.2001